Wellsim Biomedical Technologies Inc

Multi-omics Profiling of Individual Exosomes for Origin-tracing, Biomarker Discovery, and Biological Function Characterization - Project Summary / Abstract Extracellular Vesicles (EVS) Exhibit High Heterogeneity in Biofluids, a Feature That Traditional Bulk-level Analysis Approaches Fail to Capture in Terms of Individual Variations. While Numerous Techniques Exist for Single-ev Analysis, the Majority Focus Primarily on Profiling Surface Proteins. Transcriptional Analysis at the Level of Individual Evs, However, Remains Largely Unexplored. to Bridge This Gap, We Propose the Development of a Technology for Multimodal Profiling of Individual Evs, Leveraging Next-generation Sequencing and an Optimized Method for Multiplex Library Preparation. This Proposed Platform Will Serve as a Unique Tool for High-throughput, Integrative Profiling of Single-ev Gene Expression and Surface Proteins. It Aims to Offer High-sensitivity, Multi- Dimensional Biological Insights, Thereby Potentially Accelerating the Advancement of Ev-based Diagnostics and Targeted Therapies.