Electronic Biosciences, Inc.

Dual Reader Protein Sequencing - Project Summary as It Stands Today, Traditional/standard Protein Characterization Methods Have Insufficient Limits-of-detection, Dynamic Range, Throughput, Cost, Accuracy, Sensitivity, Scale, And/or Some Combination Thereof. Because of These Shortcomings, There Are No Currently Available Methods Capable of Meeting the Needs Within the Proteomics Field: Single-cell, Proteome-wide Characterization/sequencing. New Technology Must Be Developed to Advance and Revolutionize the Field of Proteomics, Similar to How Inventive Nanopore-based Technology Developments Have Opened and Accelerated the Fields of Genomics and Transcriptomics. Nanopore-based Technology Is a Very Powerful Method for Molecular Characterization and Because of This, It Has the Potential to Also Shape the Future of Protein Sequencing. It Is One of Only a Few Potential Approaches That Represent a Viable Path to Direct, High-throughput, High-sensitivity, Single-molecule, Protein Sequencing Capable of Characterizing Both Low- and High-abundance Proteins, Which Is an Absolute Necessity for Achieving the Accuracy and Dynamic Range Required for Comprehensive, Enabling Protein Analyses. During This Program, Electronic Biosciences, Inc. (EBS) Aims to Develop a Completely New Nanopore-based Technology That Will Enable De Novo Protein Sequencing. During This Phase I Project, We Will Develop and Build a Novel Protein Sequencing System Prototype, Fully Assess and Optimize the Associated Workflow/methodology for Highly Controlled and Versatile Protein/peptide Characterization, and Demonstrate Initial Sequencing for Various Proteins and Peptides. at the Conclusion of This Project, We Will Have Successfully Shown Concept Feasibility for Practical Nanopore-based Protein Sequencing.