Atgc Inc

Novel Methods to Improve Nuclease Mediated Homologous Recombination, Administrative Suppl - Project Abstract in This Administrative Supplement Grant Application, We Request Funds to Purchase a New Cell Sorter (S3E™ Cell Sorter, Bio-rad). This State of Art Cell Sorter Will Enable Us to Evaluate Large Size Gene Knock-in Events, Including Both On-target Knock-in, On-target Insertion/deletion (INDEL) Rates as Well as Potential Off-target Knock-in and Indel Events, at the Single Cell Resolution. in Aim 1, We Plan to Use This Cell Sorter to Evaluate MI-SPCAS9-V2. in Aim 2, We Will Use This Cell Sorter to Evalute MI-SACAS9. in Aim 3, We Will Use This Cell Sorter to Evaluate the Efficacy and Safety Profiles of MICAS9 in Clinically Relevant Applications. as a Results, This New Cell Sorter, Once Equipped, Will Generate More Convincing Data to Demonstrate the Advantages of the Proposed MICAS9 Gene Editing Nucleases.