Max Planck Florida Corp

Identifying Neurons for Interoception Using Simultaneous Profiling of Activity- and Projection- Specific Populations - Project Summary Interoception, the Process by Which the Body Senses Its Own Internal State, Is Critical to Maintaining Homeostasis Through the Detection of Physiological Changes That Enable the Body to Adjust to Changing Demands. Dysfunction in Interoception May Lead to Erroneous Prediction Errors Concerning These Bodily Needs and Is Increasingly Considered to Underlie a Number of Maladaptive Behaviors and Psychiatric Disorders, Including Addiction and Eating Disorders. Despite This, Little Progress Has Been Made in Identifying the Underlying Neural Circuit Mechanisms of Interoception Because Non-verbal Subjects (e.g. Animal Models) Cannot Self-report Internal States. Here, We Propose a Novel Conceptual Behavioral Framework for Studying Interoception in Animal Models in Order to Identify Neuronal Ensembles That Encode Interoception. Moreover, Human Imaging Studies Have Informed Us That Interoception Relies Critically on an Understudied Area of the Brain, the Insular Cortex, But the Functions and Corresponding Projections From the Insular Cortex Subregions (anterior to Posterior) Have Not Been Well-studied. Methods That Can Simultaneously Deliver Precise Information Concerning Behavior and Projections in a High-throughput Way Are Therefore Required. Molecular Profiling Techniques Have Been Increasingly Useful for Identifying Cell Types That Might Serves as the Link Between Genes to Circuits, But Current Techniques Have Limitations, Namely the Modality by Which the Profiling Occurs. We Therefore Also Propose a New Transcriptomic Molecular Profiling Technique, Called Snap-trap (simultaneous Neuronal Activity and Projection – Translating Ribosome Affinity Purification), That Enables Coincident Profiling of Both Activity-dependent and Projection-specific Neuronal Markers. We Will Validate This Technique Using a Well-defined Neural Circuit With Known Molecular Markers and Behavioral Consequences. We Will Then Apply the Methodology to the Insular Cortex and Its Role in Interoception. This Technique Will Also Enable Us to Make Comparisons of Next-generation Rna-sequencing to Single- Cell Rna Sequencing for the Purpose of Identifying Useful Markers for Behavioral Validation. Lastly, We Will Map Our Findings Back Onto Tissue Sections to Achieve Spatial Transcriptomic Information. Through These Experiments We Hope to Achieve a Comprehensive Transcriptomic Map of the Insular Cortex That Can Be Precisely Delineated According to Particular Behaviors and Projections, and Can Be Used as a Basis for Understanding How Dysfunction in Interoception Leads to Maladaptive Behaviors. the Snap- Trap Technique May Then Be Used by the Broader Neuroscience Community in Other Brain Regions and Behavioral Tasks to Gain Insights Into the Neural Underpinnings of Complex Behaviors and Their Associated Psychiatric Disorders.