Creative Biolabs Inc

Identification of Host Proteins That Interact With Asfv Proteins Through Yeast Two Hybrid Screening - African Swine Fever (ASF) Is a Large Dna Virus of Approximately 180KB, and Contains Approximately 160 Different Open Reading Frames (orf), With Little or No Function. the Primary Target Cell in Swine Is Macrophages. to Identify a Known Function for These Genes One Methodology Is to Identify the Host Proteins That Interact With the Various Asfv Orfs. However, Currently There Is No Yeast Library That Is Derived From Swine Macrophage Cdna. to Further Complicate Things Many of the Asfv Proteins Have Membrane Localization or Membrane Insertion Signals. Membrane Proteins Are Very Difficult to Study Protein-protein Interactions. a System, Using for Identifying Membrane Protein Interactions, Is Needed That Is Compatible for Use for Swine Proteins. the Goal of This Project Is to Design a Complete Genome Membrane Yeast Two Hybrid System Compatible to Identify Protein Interactions in a Swine System, and Validate These Interactions Do Occur in Mammalian Cells, and During Viral Infection.